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Rabbit Anti-PLIN2 antibody
Rabbit Anti-PLIN2 antibody
PLIN2_HUMAN; Perilipin-2; ADFP; Adipophilin; Adipose differentiation-related protein;
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Details

Product Name PLIN2
Chinese Name 脂肪分化相关蛋白Recombinant rabbit monoclonal anti
Alias PLIN2_HUMAN; Perilipin-2; ADFP; Adipophilin; Adipose differentiation-related protein;   
Research Area Tumour  Cardiovascular  Signal transduction  
Immunogen Species Rabbit
Clonality Monoclonal
React Species (predicted: Human, Mouse, )
Applications WB=1:500-1000 ICC=1:20-100 IF=1:20-100 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 48kDa
Cellular localization The cell membrane 
Form Liquid
Concentration 1mg/ml
immunogen Recombinant human PLIN2 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Function:
May be involved in development and maintenance of adipose tissue.

Subcellular Location:
Membrane; Peripheral membrane protein.

Tissue Specificity:
Milk lipid globules.

Post-translational modifications:
Acylated; primarily with C14, C16 and C18 fatty acids.

Similarity:
Belongs to the perilipin family.

SWISS:
Q99541

Gene ID:
123

Database links:

Entrez Gene: 123 Human

Entrez Gene: 11520 Mouse

SwissProt: Q99541 Human

SwissProt: P43883 Mouse

SwissProt: Q4PLW0 Pig




Product Picture
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: Mouse brain, 2: Rat brain
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 48 kDa
Observed MW: 48 kDa
Cell line: HepG2
Fixation: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation condition: 4°C
overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for
SLM-54764R

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