Product Name	NOX2
Chinese Name	NOX2Recombinant rabbit monoclonal anti
Alias	AMCBX2; CGD; CGD91-phox; CY24B_HUMAN; CYBB; Cytochrome b 245, beta polypeptide; Cytochrome b(558) beta chain; Cytochrome b(558) subunit beta; Cytochrome b-245 heavy chain; Cytochrome b558 subunit beta; GP91 PHOX; gp91-1; gp91-phox; GP91PHOX; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; p22 phagocyte B-cytochrome; P91 PHOX; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit.
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	9B12
React Species	Human,  (predicted: Mouse, Rat, )
Applications	WB=1:300-1000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	65kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human NOX2: 150-250/570
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	NOX2/gp91phox is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD). X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections. Function: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Subcellular Location : Membrane. Post-translational modifications: Glycosylated. DISEASE: Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Similarity: Contains 1 FAD-binding FR-type domain. Contains 1 ferric oxidoreductase domain. SWISS: P04839 Gene ID: 1536 Database links: Entrez Gene: 281112 Cow Entrez Gene: 1536 Human Entrez Gene: 13058 Mouse Entrez Gene: 100008801 Rabbit Entrez Gene: 66021 Rat Omim: 300481 Human SwissProt: O46522 Cow SwissProt: P04839 Human SwissProt: Q61093 Mouse Unigene: 292356 Human Unigene: 200362 Mouse NOX2也是一个很重要的产生氧自由基的酶。主要存在于甲状腺,前列腺，唾液腺和直肠粘膜中。
Product Picture	Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: Hela (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti- NOX2 (SLM-52603R) at 1/1000 dilution Anti-beta-Actin (SL0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58-65 kD Observed band size: 58 kD Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HepG2, 2: MCF-7, 3: Mouse spleen, 4: Rat spleen Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 65 kDa Observed MW: 60 kDa

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