Product Name	Histone H2A.Z
Chinese Name	组蛋白H2AZRecombinant rabbit monoclonal anti
Alias	H2A histone family member Z; H2A.z; H2A/z; H2AFZ; H2AZ; MGC117173; H2AZ_HUMAN.
Research Area	Cell biology  Developmental biology  Epigenetics
Immunogen Species	Rabbit
Clonality	Monoclonal
React Species	Human,  (predicted: Mouse, Rat, )
Applications	WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	13kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human Histone H2A.Z protein (1-100aa)
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]. Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Subcellular Location: Nucleus. Chromosome. Post-translational modifications: Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression. Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis (By similarity). Not phosphorylated. Similarity: Belongs to the histone H2A family. SWISS: P0C0S5 Gene ID: 3015 Database links: Entrez Gene: 3015 Human Entrez Gene: 51788 Mouse Entrez Gene: 58940 Rat Omim: 142763 Human SwissProt: P0C0S5 Human SwissProt: P0C0S6 Mouse SwissProt: P0C0S7 Rat Unigene: 119192 Human Unigene: 117541 Mouse Unigene: 112573 Rat
Product Picture	Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human MCF-7 cell lysates Lane 4: Human U251 cell lysates Primary: Anti-Histone H2A.Z (SLM-52098R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13 kDa Observed band size: 15 kDa Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H2A.Z) Monoclonal Antibody, Unconjugated (SLM-52098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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