Product Name	5T4
Chinese Name	滋养层细胞glycoprotein5T4Recombinant rabbit monoclonal anti
Alias	5T4; 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; 5T4AG; M6P1; TPBG; TPBG_HUMAN; Trophoblast glycoprotein.
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	23B1
React Species	(predicted: Human, )
Applications	WB=1:500-1000 IP=1:50-100 IHC-P=1:1000-2000 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The cell membrane
Form	Lyophilized or Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TPBG/5T4
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	TPBG is a 420 amino acid single-pass type I membrane protein expressed by all types of trophoblasts as early as 9 weeks of development. TPBG contains an N-terminal putative signal sequence, a 310-residue extracellular region, a membrane anchorage domain and a 44-amino acid cytoplasmic tail with a potential phosphorylation site. The extracellular region has seven potential N-glycosylation sites and seven leucine-rich repeats, which are located in two regions separated by a hydrophilic stretch. Suggested to be involved in cell adhesion, TPBG may also be associated with tumor growth and progression. Subcellular Location: Membrane. Tissue Specificity: Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma. Similarity: Contains 6 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: Q13641 Gene ID: 7162 Database links: Entrez Gene: 7162 Human Entrez Gene: 21983 Mouse Entrez Gene: 83684 Rat Omim: 190920 Human SwissProt: Q13641 Human SwissProt: Q9Z0L0 Mouse SwissProt: Q5PQV5 Rat Unigene: 82128 Human Unigene: 20864 Mouse Unigene: 484180 Mouse Unigene: 43122 Rat
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: MCF-7, 2: JAR Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 46 kDa Observed MW: 70-80/85 kDa Tissue: Human placenta Section type: Formalin-fixed & Paraffin - embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-52422R IP of MCF-7 cells extracts IP ab incubation condition: PTM-6392, 4°C overnight, 1:100 dilution WB primary ab incubation condition: SLM-52422R , room temperature 2h, 1:1000 dilution Secondary ab: Anti-Rabbit IgG for IP (HRP) Blocking buffer and concentration: 5% NFDM/TBST Diluting buffer and concentration: 5% NFDM/TBST 1: 5% Input 2: IP with SLM-52422R 3: IP with Rabbit monoclonal IgG Isotype Observed MW: 70-80/85 kDa Exposure time: 120 s

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