Product Name	COX4I1
Chinese Name	细胞色素c氧化酶IVRecombinant rabbit monoclonal anti
Alias	COX41_HUMAN; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1 (COX IV-1); COX-IV; COX IV; COX 4; COX 4I1;
Research Area	Cell biology  immunology  Mitochondrion
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	R8F6
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Cellular localization	cytoplasmic The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human COX IV
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008] Subcellular Location: Mitochondrion inner membrane. Tissue Specificity: Ubiquitous. SWISS: P13073 Gene ID: 1327 Database links: Entrez Gene: 1327 Human Entrez Gene: 12857 Mouse Entrez Gene: 29445 Rat SwissProt: P13073 Human SwissProt: P19783 Mouse SwissProt: P10888 Rat
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Jurkat, 2: MEF, 3: C6, 4: Mouse heart Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 20 kDa Observed MW: 15 kDa Tissue: Human Kidney Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60712R Tissue: Human liver Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60712R

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