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Product Name HSD17B1 Chinese Name 羟类固醇脱氢酶17β-HSDRecombinant rabbit monoclonal anti Alias 11beta-HSD1; 17beta-HSD1; 17 beta HSD 1; 17 beta hydroxysteroid dehydrogenase type 1; 17β-hydroxysteroid dehydrogenasetype 1 EDH17B2; EDHB17; Estradiol 17 beta dehydrogenase 1; HSD17; Hydroxysteroid (17 beta) dehydrogenase 1; Placental 17 beta hydroxysteroid dehydrogenase; DHB1_HUMAN. Research Area Signal transduction Immunogen Species Rabbit Clonality Monoclonal Clone NO. R6D3 React Species (predicted: Human, ) Applications WB=1:1000-5000 IHC-P=1:100-500 IHC-F=1:100-400 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 35kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human HSD17B1 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
Subcellular Location:
Cytoplasm.
SWISS:
P14061
Gene ID:
3292
Database links:Entrez Gene: 3292 Human
Omim: 109684 Human
SwissProt: P14061 Human
Unigene: 654385 Human
Unigene: 655222 Human
Product Picture Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:20000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: Human heart (Negative control), 2:
Human placenta
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 35 kDa
Observed MW: 35 kDa
Tissue: Human prostatic hyperplasia (Negative
tissue)
Section type: Formalin-fixed &
Paraffin-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: No staining on SLM-60664R
Tissue: Human placenta
Section type: Formalin-fixed &
Paraffin-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
SLM-60664R
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