Product Name	Lysozyme
Chinese Name	溶菌酶Recombinant rabbit monoclonal anti
Alias	1 4 beta n acetylmuramidase c; 14 beta N acetylmuramidase; 14 beta N acetylmuramidase C; EC 3.2.1.17; lysosyme; Lysozyme C; Lysozyme C precursor; Lyz; LZM; Muramidase; LYSC_HUMAN.
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	8F12
React Species	(predicted: Human, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:400-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Detection molecular weight	13 kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Recombination human Lysozyme: 1-130/130
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014] Subcellular Location: Secreted. SWISS: P61626 Gene ID: 4069 Database links: Entrez Gene: 4069 Human Omim: 153450 Human SwissProt: P61626 Human Unigene: 524579 Human
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa (Negative control), 2: THP-1 Protein loading quantity: 20 ug Exposure time: 60 s Predicted MW: 17 kDa Observed MW: 13 kDa Tissue: Human tonsil Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60643R Tissue: Human spleen Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60643R

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