Product Name	Alpha Tubulin (Acetyl-Lys40)
Chinese Name	乙酰化微管蛋白α/Tubulin αRecombinant rabbit monoclonal anti
Alias	Alpha tubulin 1; Alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; TBA1A_HUMAN; TBA1B_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1 (testis specific); Tubulin alpha 1; Tubulin alpha 1 chain; Tubulin alpha 4a; Tubulin alpha 4A chain; Tubulin alpha-1 chain; Tubulin alpha-4A chain; Tubulin H2 alpha; Tubulin H2-alpha; TUBA4A; α tubulin.
Product Type	Acetylated anti Recombinant rabbit monoclonal anti
Research Area	Cell biology  Chromatin and nuclear signals  Epigenetics
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	R2A9
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:1000-4000 IHC-P=1:100-500 IHC-F=1:50-100 ICC=1:25 IF=1:25 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Alpha Tubulin (Acetyl-Lys40): SD(Ac-K)TI
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] Subcellular Location: Cytoplasm, cytoskeleton. Tissue Specificity: Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex SWISS: P68366 Gene ID: 7277 Database links: Entrez Gene: 7277 Human Entrez Gene: 22145 Mouse Entrez Gene: 316531 Rat SwissProt: P68366 Human SwissProt: P68368 Mouse SwissProt: Q5XIF6 Rat
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:6000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Mouse brain, 2: Rat brain Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 50 kDa Observed MW: 55 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): MCF-7+serum starvation(14hr), (+): MCF-7+ sodium butyrate (50mM, 24hr) + trichostain A (500ng/ml, 4 hr) Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 50 kDa Observed MW: 55 kDa Tissue: Rat cerebellum Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60706R Tissue: Human cerebrum Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60706R

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