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Product Name DUSP5 Chinese Name 双特异性磷酸酶5Recombinant rabbit monoclonal anti Alias Dual specificity protein phosphatase 5; Dual specificity protein phosphatase hVH 3; Dual specificity protein phosphatase hVH3; DUSP 5; DUSP; HVH 3; HVH3; Serine/threonine specific protein phosphatase; VH 3; VH1 like phosphatase 3; VH3; DUS5_HUMAN. Research Area Tumour Cell biology Neurobiology Signal transduction Apoptosis Kinases and Phosphatases Immunogen Species Rabbit Clonality Monoclonal Clone NO. R1A10 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:200-1000 IHC-P=1:100-500 IHC-F=1:200-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 42kDa Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DUSP5 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq, Jul 2008]
Subcellular Location:
Nuclear.
SWISS:
Q16690
Gene ID:
1847
Database links:
Entrez Gene: 1847 Human
Entrez Gene: 240672 Mouse
Omim: 603069 Human
SwissProt: Q16690 Human
Product Picture Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HCT-116, 2: Raji, 3: K562, 4: C2C12,
5: PC12
Protein loading quantity: 20 ug
Exposure time: 60 s
Predicted MW: 42 kDa
Observed MW: 42 kDa
Tissue: Human papillary thyroid carcinoma
Section type: Formalin-fixed &
Paraffin-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
SLM-60646RTissue: Human bladder carcinoma
Section type: Formalin-fixed &
Paraffin-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
SLM-60646RTissue: Human bladder carcinoma
Section type: Formalin-fixed &
Paraffin-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
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