Product Name	NGFR/p75NTR
Chinese Name	神经生长因子受体Recombinant rabbit monoclonal anti
Alias	TNR16_HUMAN; Tumor necrosis factor receptor superfamily member 16; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor (NGF receptor 1 Publication); p75 ICD; CD271; TNFRSF16; p75NTR; p75(NTR); Gp80-LNGFR; p75 NGF Receptor;
Research Area	Cell biology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	5D4
React Species	(predicted: Human, Rat, )
Applications	WB=1:1000-5000 IHC-P=1:100-500 ICC=1:50-200 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	44kDa
Cellular localization	The nucleus cytoplasmic The cell membrane Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human p75 NGF Receptor
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells. NGF is important for the development, differentiation and survival of a variety of neuronal and non-neuronal cells. Its action is mediated by binding to two distinct receptors, the high affinity p140 and low affinity p75. p75NGFR binds neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, and NT-6. p75NGFR belongs to the TNF-R superfamily and is reported to mediate NGF-induced apoptosis. Function: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Subunit: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with LINGO1 (By similarity). Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with NRADD. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: N- and O-glycosylated. Phosphorylated on serine residues. Similarity: Contains 1 death domain. Contains 4 TNFR-Cys repeats. SWISS: P07174 Gene ID: 4804 Database links: Entrez Gene: 4804 Human Entrez Gene: 18053 Mouse Entrez Gene: 24596 Rat Omim: 162010 Human SwissProt: P08138 Human SwissProt: Q9Z0W1 Mouse SwissProt: P07174 Rat Unigene: 415768 Human Unigene: 681726 Human Unigene: 283893 Mouse Unigene: 10980 Rat Neurobiology相关蛋白（Neurobiology） NGFR神经生长因子受体又称：CD271存在与细胞上的与神经生长因子相结合并相互作用的蛋白质因子，属一型跨膜TNF受体超家族glycoprotein，它可与NGF, BDNF, NT-3, NT-4 结合，根据与神经生长因子的结合力速度及亲和力可分为高亲和力和低亲和力两种NGFR。 此抗体识别分子量为47kDa低亲和力的NGFR.NGF-R也称为低亲和神经生长因子受体，属1型跨膜TNF受体超家族。
Product Picture	Tissue: Human prostatic hyperplasia Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-52253R Tissue: Human tonsil Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-52253R Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: SW480, 2: PC-12 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 45 kDa Observed MW: 70 kDa Cell line: PC-12 Fixation: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-52253R Cell line: PC-12 Fixation: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary Ab dilution: 1:100 Secondary Ab: Goat Anti-Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for SLM-52253R

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