Product Name	N Cadherin
Chinese Name	N-钙粘附分子Recombinant rabbit monoclonal anti
Alias	Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; cadherin 2 type 1 N-cadherin neuronal; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; NCAD; Neural Cadherin; Neural Cadherin.
Research Area	Tumour  Cell biology  immunology  Kinases and Phosphatases  Cell adhesion molecule
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	5G1
React Species	Human, Mouse, Rat,
Applications	WB=1:1000-5000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:400-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	100kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human N Cadherin: 150-250/905
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. SWISS: P19022 Gene ID: 1000 Database links: Entrez Gene: 1000 Human Entrez Gene: 281062 Cow Entrez Gene: 12558 Mouse Entrez Gene: 83501 Rat Omim: 114020 Human SwissProt: P19022 Human SwissProt: P15116 Mouse SwissProt: Q9Z1Y3 Rat Unigene: 464829 Human Unigene: 606106 Human Unigene: 257437 Mouse Unigene: 23200 Rat
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: HeLa, Mouse brain, Rat brain Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 100 kDa Observed MW: 125 kDa Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Human PANC-1 cell lysates Lane 5: Human A549 cell lysates Primary: Anti-N Cadherin (SLM-52389R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kDa Observed band size: 140 kDa Tissue: Human endometrium Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-52389R Tissue: Human liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-52389R Tissue: Rat liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-52389R Tissue: Mouse liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-52389R

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