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Product Name SOX2 Chinese Name 胚胎Stem cells关键蛋白重组兔单克隆抗体 Alias transcriptional factor SOX2; ANOP3; cb236; Delta EF2a; lcc; MCOPS3; MGC148683; MGC2413; RGD1565646; Sex determining region Y box 2; Sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY box containing gene 2; SRY related HMG box 2; SRY related HMG box gene 2; SRY-box 2; ysb; SOX2_HUMAN; Transcription factor SOX-2; SOX2_HUMAN. Immunogen Species Rabbit Clonality Monoclonal Clone NO. D1H6 React Species (predicted: Human, Mouse, Rat, ) Applications
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 34kDa Cellular localization The nucleus Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SOX2 Lsotype IgG Purification affinity purified by Protein A Buffer Solution PBS, Glycerol, BSA Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008].
Function:
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation.
Subunit:
Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.
Subcellular Location:
Nucleus.
Post-translational modifications:
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
DISEASE:
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Similarity:
Contains 1 HMG box DNA-binding domain.
SWISS:
P48432
Gene ID:
6657
Database links:Entrez Gene: 6657 Human
Entrez Gene: 20674 Mouse
Omim: 184429 Human
SwissProt: P48431 Human
SwissProt: P48432 Mouse
Unigene: 518438 Human
Unigene: 65396 Mouse
Embryonic Stem Cell Marker (胚胎Stem cellsMaker)
转录因子:胚胎Stem cells相关蛋白Sox2是sox基因家族的一个成员,Sox2与Oct4、Nanog一样是胚胎Stem cells重要的转录因子,是维持Stem cells特性中起到重要的作用因子;由于它在早期胚胎发生、神经分化和晶状体发育等多种重要的发育事件中都起着关键的作用,从而引起了越来越广泛的关注。Product Picture Paraformaldehyde-fixed, paraffin embedded (rat embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOX2) Monoclonal Antibody, Unconjugated (SLM-60788R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOX2) Monoclonal Antibody, Unconjugated (SLM-60788R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOX2) Monoclonal Antibody, Unconjugated (SLM-60788R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOX2) Monoclonal Antibody, Unconjugated (SLM-60788R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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