Product Name	[KO validated anti] NRSF
Chinese Name	神经元抑制蛋白抗体
Alias	RE1-Silencing Transcription Factor; NRSF; Neural-Restrictive Silencer Factor; XBR; Neuron Restrictive Silencer Factor; Repressor Binding To The X2 Box; X2 Box Repressor; REST_HUMAN
literatures	Specific References  (2)     |     SL2590R has been referenced in 2 publications. [IF=9.889] Buqing Yeet al. The chromatin remodeler SRCAP promotes self‐renewal of intestinal stem cells. EMBO J . 2020 Jul 1;39(13):e103786.  IHC, IP, IF ;  mouse.   PubMed:32449550 [IF=2.472] Zhu NW et al. Possible mechanisms of lycopene amelioration of learning and memory impairment in rats with vascular dementia. Neural Regen Res. 2020 Feb;15(2):332-341.  WB&IHC-P ;  Rat.   PubMed:31552907
Research Area	Neurobiology  Zinc finger protein  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	121kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NRSF: 401-450/1097
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010] Function: Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. Subunit: Interacts with SIN3A, SIN3B and RCOR1. Interacts with CDYL. Interacts with EHMT1 and EHMT2 only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. Similarity: Contains 9 C2H2-type zinc fingers. SWISS: Q13127 Gene ID: 5978 Database links: Entrez Gene: 5978 Human Entrez Gene: 19712 Mouse Entrez Gene: 83618 Rat GenBank: AAB17211 Human Omim: 600571 Human SwissProt: Q13127 Human SwissProt: Q155C6 Mouse SwissProt: Q8VIG1 Mouse SwissProt: O54963 Rat SwissProt: O54964 Rat Unigene: 307836 Human Unigene: 631513 Human Unigene: 28840 Mouse Unigene: 482359 Mouse Unigene: 10879 Rat
Product Picture	Sample: Hela(Human) Cell Lysate at 30 ug Hela KO NRSF (Human) Cell Lysate at 30 ug Primary: Anti- NRSF (SL2590R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-NRSF (SL2590R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD NRSF was immunoprecipitated from human hela cells lysate with SL2590R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution. Lane 1: human hela cells lysate 10 µg (Input). Lane 2: SL2590R IP in human hela cells lysate. Lane 3: native rabbit IgG IP in human hela cells lysate (negative control). Secondary All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000 Tissue/cell: human gastric tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated(SL2590R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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