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Product Name NAPSIN A Chinese Name 天冬氨酸蛋白酶ASP4抗体 Alias NAPSIN A; Asp 4; ASP4; Aspartyl protease 4; KAP; Kdap; Kidney derived aspartic protease like protein; NAP1; NAPA; Napsa; NAPSA_HUMAN; Napsin 1; napsin A aspartic peptidase; Napsin A precursor; Napsin-1; Napsin-A; Pronapsin A; SNAPA; TA01/TA02. Immunogen Species Mouse Clonality Monoclonal Clone NO. 10A12 Applications
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 39kDa Cellular localization Secretory protein Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse NAPSIN A Lsotype IgG1/kappa Purification affinity purified by Protein A Buffer Solution Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Napsin A is an aspartic proteinase that belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. It is a 420-amino acid polypeptide consisting of a 24-residue signal peptide, a 40-amino acid propart, the mature enzyme of 336 amino acids, and a C-terminal extension of 18 residues. The mature Napsin A protein contains 3 predicted disulfide bonds, 3 potential N-linked oligosaccharide attachment sites, an RGD motif, a recognition motif for integrin binding, in the C terminus, immediately before a 4-amino acid insert that is unique to aspartic proteinases. Highest levels of Napsin A have been detected in adult lung (type II pneumocytes), fetal lung, and kidney tissues. Napsin A is also expressed at lower levels in adult spleen and at very low levels in peripheral blood leukocytes. Human napsin A shares 72.6% sequence identity with the mouse homolog.
Function:
May be involved in processing of pneumocyte surfactant precursors.
Subcellular Location:
Secreted (Potential).
Tissue Specificity:
Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
Similarity:
Belongs to the peptidase A1 family.
SWISS:
O09043
Gene ID:
16541
Database links:Entrez Gene: 9476 Human
Omim: 605631 Human
SwissProt: O96009 Human
Unigene: 512843 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NAPSIN A ) Monoclonal Antibody, Unconjugated (SLM-34153M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NAPSIN A ) Monoclonal Antibody, Unconjugated (SLM-34153M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human lung ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NAPSIN A ) Monoclonal Antibody, Unconjugated (SLM-34153M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NAPSIN A ) Monoclonal Antibody, Unconjugated (SLM-34153M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NAPSIN A ) Monoclonal Antibody, Unconjugated (SLM-34153M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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