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Product Name Nestin Chinese Name 巢蛋白/神经上皮Stem cells蛋白单克隆抗体 Alias ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_HUMAN; Nestin. Immunogen Species Mouse Clonality Monoclonal Clone NO. E2G4 React Species (predicted: Human, Mouse, ) Applications
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 178kDa Cellular localization cytoplasmic Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Nestin Lsotype IgG2a κ Purification affinity purified by Protein G Buffer Solution PBS, Glycerol, BSA Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
cytoplasm
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:Entrez Gene: 10763 Human
Entrez Gene: 18008 Mouse
Omim: 600915 Human
SwissProt: P48681 Human
SwissProt: Q6P5H2 Mouse
Unigene: 527971 Human
Unigene: 331129 Mouse
Unigene: 9701 Rat
神经Stem cellsMaker在动物体早期发育过程中,中枢神经系统发育尤为重要 。Nestin是一种中等纤维蛋白,它在哺乳动物神经前体细胞中表达较高,目前已被广泛用于神经前体细胞的重要标志分子。研究Nestin的表达调控规律,将有助于人们认识神经前体细胞的生物特性。此抗体还表达中枢神经系统Stem cells及神经epithelial cells。Product Picture Paraformaldehyde-fixed, paraffin embedded (human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (SLM-60784M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human spleen ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (SLM-60784M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human brain ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (SLM-60784M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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