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Mouse Anti-RuBisCO antibody
Mouse Anti-RuBisCO antibody
rbcL; RBL_SPIOL; Ribulose bisphosphate carboxylase large chain; RuBisCO-large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit.
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  • NO.:SLM-33226M
    Clonality:Monoclonal
    Immunogen Species:Mouse
    React Species:Arabidopsis Thaliana,
    Applications:WB
    concentration:1mg/ml
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Details

Product Name RuBisCO
Chinese Name 核糖二磷酸羧化酶大链单克隆抗体
Alias rbcL; RBL_SPIOL; Ribulose bisphosphate carboxylase large chain; RuBisCO-large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit.  
Product Type Botany抗体 
Research Area Tumour  Cell biology  Signal transduction  Botany  The new supersedes the old  
Immunogen Species Mouse
Clonality Monoclonal
Clone NO. 3G5
React Species Arabidopsis Thaliana, 
Applications WB=1:500-2000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 52kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen D-Ribulose 1,5-Diphosphate Carboxylase purified 
Lsotype IgG
Purification affinity purified by Protein G
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.

Function:
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.

Subunit:
Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers.

Subcellular Location:
Plastid, chloroplast.

Post-translational modifications:
The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.

Similarity:
Belongs to the RuBisCO large chain family. Type I subfamily.

SWISS:
P00875

Gene ID:
2715621

Database links:

Entrez Gene: 2715621 Spinacia oleracea

SwissProt: P00875 Spinacia oleracea




Product Picture
Sample:
Lane 1: Brassica oleracea L. leaves (Plant) lysates
Lane 2: Chinese cabbage leaves (Plant) lysates
Primary: Anti-RuBisCO (SLM-33226M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa

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