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Product Name C-jun Chinese Name 原癌基因蛋白/活化蛋白1单克隆抗体 Alias Transcription factor AP-1; Jun oncogene; JUN; AP 1; AP1; AP-1; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN protein; JUNC; p39; Proto oncogene cJun; Transcription Factor AP1; V jun avian sarcoma virus 17 oncogene homolog; vJun Avian Sarcoma Virus 17 Oncogene Homolog; JUN_HUMAN; Activator 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog. Research Area Tumour Cell biology Signal transduction transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Mouse Clonality Monoclonal React Species Human, (predicted: Mouse, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 ICC=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 36kDa Detection molecular weight 43/36 kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen Recombinant human C-jun protein : 1-127/331 Lsotype IgG Purification affinity purified by Protein A Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.
SWISS:
P05412
Gene ID:
3725
Product Picture Sample:
Lane 1: Human 293T cell lysates
Lane 2: Recombinant human C-jun protein
Primary: Anti-C-jun (SLM-42049M) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 37,62 kDa
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (C-jun) monoclonal Antibody, Unconjugated (SLM-42049M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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