Product Name	GART
Chinese Name	甘氨酰胺核苷酸合成酶单克隆抗体
Alias	5'-phosphoribosylglycinamide transformylase; AIR synthase; AIRS; GAR transformylase; GARS; GARTF; Glycinamide ribonucleotide synthetase; MGC47764; PAIS; PGFT; Phosphoribosyl-aminoimidazole synthetase; Phosphoribosylglycinamide formyltransferase; Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase; Phosphoribosylglycinamide formyltransferase, EC 2.1.2.29; Phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase; Phosphoribosylglycinamide synthetase; PRGS; PUR2_HUMAN; Trifunctional purine biosynthetic protein adenosine 3.
Research Area	Tumour  Cell biology  Signal transduction  The new supersedes the old  Epigenetics
Immunogen Species	Mouse
Clonality	Monoclonal
React Species	Human,  (predicted: Rat, )
Applications	WB=1:500-1000 ICC=1:50  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	107kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GART
Lsotype	IgG1,Kappa
Purification	affinity purified by Protein A
Buffer Solution	PBS, Glycerol, BSA
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Purines are critical for energy metabolism, cell signaling and cell reproduction and also function as precursors for coenzymes, energy transfer molecules, regulatory factors and proteins involved in RNA and DNA synthesis. GART (GAR transformylase), also referred to as AIRS, GARS, PAIS, PGFT, PRGS or GARTF, is 1,010 amino acids in length and is a key folate-dependent trifunctional enzyme with phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and AICAR (phosphoribosylaminoimidazole synthetase) activity required for de novo purine biosynthesis. Cancer cells require considerable amounts of purines to sustain their accelerated growth and GART is, therefore, a target for cancer chemotherapy. GART is highly conserved in vertebrates. Two isoforms of GART are expressed due to alternative splicing events. SWISS: P22102 Gene ID: 2618 Database links: Entrez Gene: 2618 Human Entrez Gene: 14450 Mouse Entrez Gene: 288259 Rat Omim: 138440 Human SwissProt: P22102 Human SwissProt: Q64737 Mouse Unigene: 473648 Human Unigene: 4505 Mouse
Product Picture	Sample: Lane 1: Human HeLa cell lysates Lane 2: Human A431 cell lysates Lane 3: Human HepG2 cell lysates Primary: Anti-GART (SLM-60639M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution Predicted band size: 107 kDa Observed band size: 55,130 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: HepG2, 2: HEK-293, 3: PC-12 Protein loading quantity: 20 ug Exposure time: 30 s Predicted MW: 40, 108 kDa Observed MW: 52, 120 kDa Cell line: HepG2 Fixation: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60639M

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