Product Name	GFAP
Chinese Name	胶质纤维酸性蛋白单克隆抗体
Alias	Astrocyte Marker; FLJ45472; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.
Research Area	Tumour  Cell biology  immunology  Developmental biology  Neurobiology  Signal transduction  Stem cells  Cell adhesion molecule  Cell type markers  Cytoskeleton
Immunogen Species	Mouse
Clonality	Monoclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 IHC-P=1:400-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	48kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human GFAP protein: 1-390/432
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] SWISS: P14136 Gene ID: 2670
Product Picture	Sample: Lane 1: Human U87MG cell lysates Lane 2: Human U251 cell lysates Primary: Anti-GFAP (SLM-42001M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 50 kDa Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (SLM-42001M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (SLM-42001M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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