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Product Name PGC Chinese Name 胃蛋白酶原C单克隆抗体 Alias PEPC_HUMAN; Gastricsin; EC:3.4.23.3; Pepsinogen C; PEPC; PGII; progastricsin; Immunogen Species Mouse Clonality Monoclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 42kDa Detection molecular weight 40 kDa Cellular localization Secretory protein Form Lyophilized or Liquid Concentration 1mg/ml immunogen Recombinant human PGC protein: 17-388/388 Lsotype IgG Purification affinity purified by Protein A Buffer Solution PBS (ph=7.4) Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009]
Function:
Hydrolyzes a variety of proteins.
Subcellular Location:
Secreted.
Similarity:
Belongs to the peptidase A1 family.
SWISS:
P20142
Gene ID:
5225
Database links:Entrez Gene: 5225 Human
Omim: 169740 Human
SwissProt: P20142 Human
Unigene: 1867 Human
Unigene: 9735 Rat
胃蛋白酶原C(PG-C)主要用于胃癌及其癌前病变易感性的关系。Product Picture Sample:
Lane 1: Mouse Stomach tissue lysates
Lane 2: Rat Stomach tissue lysates
Primary: Anti-PGC (SLM-43143M) at 1/1000 dilution
Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific
Predicted band size: 42 kDa
Observed band size: 39 kDa
Sample:
Lane 1: Recombinant human PGC protein, C-His(SL43143P)
Primary: Anti-PGC (SLM-43143M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 40 kDa
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGC) Monoclonal Antibody, Unconjugated (SLM-43143M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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