Product Name	Osteocalcin
Chinese Name	骨钙素单克隆抗体
Alias	Osteocalcin Propeptide; Gamma-carboxyglutamic acid-containing protein; Bone Gla-protein; BGLAP; Bone gamma carboxyglutamate protein; BGLAP; BGP; Bone gamma carboxyglutamate gla protein osteocalcin; Bone gamma carboxyglutamate protein; Bone Gla protein; Gamma carboxyglutamic acid containing protein; OC; PMF1; OSTCN_HUMAN.
Research Area	immunology
Immunogen Species	Mouse
Clonality	Monoclonal
React Species	Human,
Applications	WB=1:500-2000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	11kDa
Cellular localization	Secretory protein
Form	Liquid
immunogen	Recombinant human Osteocalcin protein: 24-100/100
Lsotype	IgG
Purification	affinity purified by Protein A
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. SWISS: P02818 Gene ID: 632 Database links: Entrez Gene: 281646 Cow Entrez Gene: 632 Human Entrez Gene: 397530 Pig Entrez Gene: 25295 Rat Omim: 112260 Human SwissProt: P02820 Cow SwissProt: P02818 Human SwissProt: Q8HYY9 Pig SwissProt: P04640 Rat Unigene: 654541 Human Unigene: 9722 Rat
Product Picture	Sample: Lane 1: Recombinant human Osteocalcin, C-hFc (HEK293)( SL43106P) Primary: Anti-Osteocalcin (SLM-43106M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 11 kDa Observed band size: 35.5 kDa Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Monoclonal Antibody, Unconjugated (SLM-43106M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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