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Product Name CD5 Chinese Name CD5单克隆抗体 Alias T-cell surface glycoprotein CD5; Lymphocyte antigen 1; Ly-1; Lyt-1; CD-5; CD5 antigen; CD 5; CD5 molecule; CD5 antigen (p56 62); CD5_HUMAN; LEU 1; LEU1; Ly12; LyA; Lymphocyte Antigen CD5; Lymphocyte antigen T1/Leu 1; Lymphocyte antigen T1/Leu-1; Lymphocyte glycoprotein T1/Leu1; OTTHUMP00000236973; p56 62; T1. Research Area immunology Cell Surface Molecule t-lymphocyte Immunogen Species Mouse Clonality Monoclonal Clone NO. 12A8 React Species Human, (predicted: Mouse, Rat, ) Applications IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CD5 Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Function:
May act as a receptor in regulating T-cell proliferation.
Subunit:
Interacts with CD72/LYB-2. Interacts with PTPN6/SHP-1.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein.
Post-translational modifications:
Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1.
Similarity:
Contains 3 SRCR domains.
SWISS:
P06127
Gene ID:
921
Database links:Entrez Gene: 921 Human
Omim: 153340 Human
SwissProt: P06127 Human
Unigene: 58685 Human
Product Picture Blank control(black line):Jurkat.
Primary Antibody (green line): Mouse Anti-CD5 antibody (SLM-33077M)
Dilution:1ug/Test;
Secondary Antibody(white blue line): Goat anti-mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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