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Product Name CD90/Thy-1 Chinese Name CD90单克隆抗体 Alias CD90 / Thy1; CD7; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy-1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN. Research Area Cell biology immunology Neurobiology Immunogen Species Mouse Clonality Monoclonal React Species Human, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 Flow-Cyt=1ug/Test (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 12kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen Recombinant Human Thy-1 protein, C-His (HEK293): 20-141/161 Lsotype IgG Purification affinity purified by Protein A Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
SWISS:
P01830
Gene ID:
24832
Database links:
Entrez Gene: 7070 Human
Entrez Gene: 21838 Mouse
Omim: 188230 Human
SwissProt: P04216 Human
SwissProt: P01831 Mouse
Unigene: 644697 Human
Unigene: 3951 Mouse
Unigene: 108198 Rat
Product Picture Sample:
Lane 1: Recombinant Human Thy-1 protein, C-His (HEK293)
Primary: Anti-CD90/Thy-1 (SLM-43534M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 26 kDa
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD90) Monoclonal Antibody, Unconjugated (SLM-43534M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Blank control:Jurkat.
Primary Antibody (green line): Mouse Anti-CD90/Thy-14 antibody (SLM-43534M)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line):Normal Mouse IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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