Product Name	Myoglobin
Chinese Name	肌红蛋白单克隆抗体
Alias	MYO; MB; MGC13548; PVALB; MYG_HUMAN; Recombinant human Myoglobin, Trx & His
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Mouse
Clonality	Monoclonal
React Species	Mouse, Rat,  (predicted: Human, )
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Cellular localization	cytoplasmic Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human Myoglobin: 1-154/154
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Myoglobin is a small heme containing protein responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis. SWISS: P02144 Gene ID: 4151
Product Picture	Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Rat Muscle tissue lysates Lane 3: Rat Heart tissue lysates Primary: Anti-Myoglobin (SLM-41256M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 15 kDa Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Myoglobin) Monoclonal Antibody, Unconjugated (SLM-41256M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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