Product Name	Peripherin
Chinese Name	外周蛋白抗体
Alias	NEF 4; NEF4; NEF-4; Neurofilament 4; PRPH; PRPH1; PRPH 1; NEF 4; Neurofilament 4 (57kD); Perf; PERI_HUMAN.
Research Area	immunology  Neurobiology  Signal transduction  Cell adhesion molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:400-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	54kDa
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human Peripherin protein: 1-470/470
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. [provided by RefSeq]. SWISS: P41219 Gene ID: 5630 Database links: Entrez Gene: 5630 Human Entrez Gene: 19132 Mouse Entrez Gene: 24688 Rat Omim: 170710 Human SwissProt: P41219 Human SwissProt: P15331 Mouse SwissProt: P21807 Rat Unigene: 37044 Human Unigene: 2477 Mouse Unigene: 11086 Rat
Product Picture	Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Spinal cord tissue lysates Primary: Anti-Peripherin (SL42004R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 58 kDa Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (SL42004R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (SL42004R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (SL42004R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (SL42004R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (SL42004R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (SL42004R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Measured by its binding ability in a indirect ELISA. Immobilized Human Peripherin protein, His Tag (Cat. SL42004P) at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human Peripherin Antibody, the EC50 is 124.3 ng/mL.

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