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Rabbit Anti-CD81 antibody
Rabbit Anti-CD81 antibody
26 kDa cell surface protein TAPA 1; 26 kDa cell surface protein TAPA-1; 26 kDa cell surface protein TAPA1; CD 81; CD81 antigen; CD81 molecule; CD81_HUMAN; CVID6; S5.7; TAPA 1; Target of the antiproliferative antibody 1; Tetraspanin 28; Tetraspanin-28; Tet
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  • NO.:SL43118R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,(predicted: Mouse,Rat,)
    Applications:WB ELISA Flow-Cyt
    concentration:1mg/ml
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Product Name CD81
Chinese Name CD81抗体
Alias 26 kDa cell surface protein TAPA 1; 26 kDa cell surface protein TAPA-1; 26 kDa cell surface protein TAPA1; CD 81; CD81 antigen; CD81 molecule; CD81_HUMAN; CVID6; S5.7; TAPA 1; Target of the antiproliferative antibody 1; Tetraspanin 28; Tetraspanin-28; Tetraspanin28; Tspan 28; Tspan-28; Tspan28  
Research Area Cell biology  immunology  Signal transduction  lymphocyte  b-lymphocyte  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human,  (predicted: Mouse, Rat, )
Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=2ug/Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 26kDa
Form Liquid
Concentration 1mg/ml
immunogen Recombinant human CD81 protein: 113-201/236 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq, Jul 2008]

SWISS:
P60033

Gene ID:
975

Product Picture
Sample:
Lane 1: Recombinant human CD81 protein, hFc (HEK293)
Primary: Anti-CD81 (SL43118R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 36 kDa
Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-CD81 antibody (SL43118R)
Dilution: 2ug/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line):Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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