Product Name	NPHS2
Chinese Name	肾小球裂孔膜蛋白PDCN抗体
Alias	nephrosis 2, idiopathic, steroid resistant; NPHS2 gene; PDCN; Podocin; SRN1; PODO_HUMAN.
Research Area	Cell biology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	42kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human Podocin protein: 125-383/383
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Podocin is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. It is a hairpin like integral membrane protein with intracellular N and C termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. SWISS: Q9NP85 Gene ID: 7827 Database links: Entrez Gene: 7827 Human Entrez Gene: 170484 Mouse Entrez Gene: 170672 Rat Omim: 604766 Human SwissProt: Q9NP85 Human SwissProt: Q91X05 Mouse SwissProt: Q8K4G9 Rat Unigene: 412710 Human Unigene: 289099 Mouse Unigene: 86433 Rat
Product Picture	Sample: Lane 1: Recombinant human Podocin, N-his Primary: Anti-NPHS2 (SL41306R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 30 kDa Measured by its binding ability in a indirect ELISA. Immobilized Human NPHS2 protein, His Tag (Cat. SL41306P) at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human NPHS2 Antibody, the EC50 is 155.5 ng/mL. Measured by its binding ability in a indirect ELISA. Immobilized Human NPHS2 protein, His Tag (Cat. SL41306P) at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human NPHS2 Antibody, the EC50 is 374.9 ng/mL.

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