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Product Name SPARC Chinese Name 富含半胱氨酸的酸性分泌蛋白抗体 Alias AA517111; Basement membrane protein 40; BM 40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic cysteine rich (osteonectin); Secreted protein acidic cysteine rich; SPRC; SPRC_HUMAN. Research Area Tumour Cell biology Signal transduction Stem cells Cyclin Cytoskeleton Extracellular matrix Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:400-800 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 33kDa Cellular localization The cell membrane Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml immunogen Recombinant human SPARC protein: 18-303/303 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011].
Function:
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Subcellular Location:
Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane.
Similarity:
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
SWISS:
P09486
Gene ID:
6678
Database links:Entrez Gene: 6678 Human
Omim: 182120 Human
SwissProt: P09486 Human
Unigene: 111779 Human
Unigene: 708558 Human
SPARC是从多方面调节细胞功能的Extracellular matrix蛋白,SPARC蛋白在人组织中广泛分布与组织重建和Tumour有关。SPARC在多种Tumour中呈高表达.Product Picture Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Mouse B16 cell lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-SPARC (SL41345R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC ) Polyclonal Antibody, Unconjugated (SL41345R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPARC ) Polyclonal Antibody, Unconjugated (SL41345R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag (Cat. SL41345P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human SPARC Antibody, the EC50 is 72.97 ng/mL.
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