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Rabbit Anti-PRL  antibody
Rabbit Anti-PRL antibody
Luteotropic Hormone; Mammotropin; OTTHUMP00000017727; PRL; Prolactin; Prolactin precursor; PRL_HUMAN
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Product Name PRL
Chinese Name 泌乳素抗体
Alias Luteotropic Hormone; Mammotropin; OTTHUMP00000017727; PRL; Prolactin; Prolactin precursor; PRL_HUMAN  
Research Area Cell biology  Signal transduction  Growth factors and hormones  Kinases and Phosphatases  Endocrinopathy  
Immunogen Species Rabbit
Clonality Polyclonal
React Species (predicted: Human, )
Applications ELISA=1:5000-10000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 24kDa
Cellular localization Secretory protein 
Form Liquid
Concentration 1mg/ml
immunogen Recombinant human PRL protein: 29-227/227 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011].

SWISS:
P01236

Gene ID:
5617

Product Picture
Sample:
Lane 1: Recombinant human PRL protein, N-His (HEK293)(SL43099P)
Primary: Anti-PRL (SL43099R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa
Paraformaldehyde-fixed, paraffin embedded (human pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRL) Polyclonal Antibody, Unconjugated (SL43099R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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