Product Name	IL-6
Chinese Name	白介素6抗体
Alias	Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; Interleukin-6; Interleukin6; IL6_HUMAN
Research Area	Tumour  Cell biology  immunology  Neurobiology  Growth factors and hormones
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	23kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human IL-6 (C-6x His-Tag): 30-212/212
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]. SWISS: P05231 Gene ID: 3569 Database links: Entrez Gene: 3569 Human Entrez Gene: 16193 Mouse Entrez Gene: 24498 Rat Omim: 147620 Human SwissProt: P05231 Human SwissProt: P08505 Mouse SwissProt: P20607 Rat Unigene: 654458 Human Unigene: 1019 Mouse Unigene: 9873 Rat
Product Picture	Sample: Lane 1: Recombinant human IL-6 protein (SL10807P) Lane 2: Recombinant human IL-6 protein (SL41177P) Primary: Anti-IL-6 (SL41177R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kDa Observed band size: 22 kDa Sample: Lane 1: Human HUVEC cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-IL-6 (SL41177R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kDa Observed band size: 28 kDa Measured by its binding ability in a indirect ELISA. Immobilized Human IL-6 protein, His Tag (Cat. SL41177P) at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human IL-6 Antibody, the EC50 is 181.7 ng/mL. Measured by its binding ability in a indirect ELISA. Immobilized Human IL-6 protein, His Tag (Cat. SL41177P) at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human IL-6 Antibody, the EC50 is 54.11 ng/mL.

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