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Rabbit Anti-Ki67  antibody
Rabbit Anti-Ki67 antibody
Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.
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  • NO.:SLM-60738R
    Clonality:Monoclonal
    Immunogen Species:Rabbit
    React Species:(predicted: Human,Mouse,Rat,)
    Applications:IHC-P ICC IF
    concentration:1mg/ml
  • Goods click count:30
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Details

Product Name Ki67
Chinese Name Ki67蛋白Recombinant rabbit monoclonal anti
Alias Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.  
literatures
Specific References  (1)     |     SLM-60738R has been referenced in 1 publications.
[IF=7.59] Guoyun Wan. et al. Endoplasmic reticulum-targeted NIR-II phototherapy combined with inflammatory vascular suppression elicits a synergistic effect against TNBC. BIOMATER SCI-UK. 2023 Jan;:  IHC ;  Mouse.  
Research Area Tumour  Cell biology  Neurobiology  Cyclin  Cell differentiation  Cell type markers  Epigenetics  
Immunogen Species Rabbit
Clonality Monoclonal
Clone NO. 6B9
React Species (predicted: Human, Mouse, Rat, )
Applications IHC-P=1:100-500 ICC=1:50-100 IF=1:50-100 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 358kDa
Cellular localization The nucleus 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human Ki67 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution PBS, Glycerol, BSA
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

SWISS:
P46013

Gene ID:
4288

Database links:

Entrez Gene: 4288 Human

Entrez Gene: 17345 Mouse

Entrez Gene: 246042 Rat

Omim: 176741 Human

SwissProt: P46013 Human

SwissProt: Q91VE6 Mouse

SwissProt: Q5RJM0 Rat

Unigene: 689823 Human

Unigene: 80976 Human

Unigene: 4078 Mouse

Unigene: 233802 Rat



Product Picture
Tissue: Rat colon
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for
SLM-60738R
Tissue: Mouse spleen
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for SLM-60738R
Tissue: Human cardiac muscle (Negative
tissue)
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: No staining on human cardiac
muscle
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for SLM-60738R
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour
at room temperature
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal
for SLM-60738R

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