Product Name	Neural Lineage Markers Antibody Assembly kit
Chinese Name	神经谱系标记抗体套装
Alias	Neural Lineage Markers Antibody Assembly kit(TUBB3,GFAP,MAP2,Nestin,NeuN,Vimentin)  神经谱系标记抗体套装,神经系表面标记抗体套装(TUBB3,GFAP,MAP2,Nestin,NeuN,Vimentin)
Research Area	Neurobiology  Cell type markers
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	IHC-P=1:100-500 IHC-F=1:400-800 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	In central nervous system, neural stem cells (NSC) differentiate into multiple neural lineages, including neural precursors, astrocytes, neurons, and oligodendrocytes. Neuronal lineage markers are endogenous tags that are expressed in different cells along neurogenesis and differentiated cells such as neurons. We can use it to detect and identify different types of cells by different techniques. Neural Lineage Markers Antibody Assembly kit is one economical package of selected antibodies for neural lineage markers, including neural stem cell and progenitor marker Nestin, mature neuron marker NeuN and MAP2, Immature neuron maker TUBB3, radial glia cell marker Vimentin, mature astrocytes and other glial cell marker GFAP. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet. Neural Lineage Markers Antibody Assembly kit Rabbit Anti-TUBB3 (Neuronal Marker) antibody (SL4512R) Rabbit Anti-GFAP antibody (SL0199R) Rabbit Anti-MAP2 antibody (SL1369R) Rabbit Anti-Nestin antibody (SL0008R) Rabbit Anti-NeuN antibody (SL1613R) Rabbit Anti-Vimentin antibody (SL0756R) SWISS: N/A Gene ID: N/A Database links: Neural Lineage Markers Antibody Assembly kit Rabbit Anti-TUBB3 (Neuronal Marker) antibody (SL4512R) Rabbit Anti-GFAP antibody (SL0199R) Rabbit Anti-MAP2 antibody (SL1369R) Rabbit Anti-Nestin antibody (SL0008R) Rabbit Anti-NeuN antibody (SL1613R) Rabbit Anti-Vimentin antibody (SL0756R) 在中枢神经系统中，神经Stem cells(NSC)可分化为多种神经细胞类型，包括神经前体、星形胶质细胞、神经元和少突胶质细胞等。神经谱系标记是一些在神经发生或分化后不同细胞类型中特征性表达的一些蛋白。我们利用可以这些特征性标记通过不同的技术来检测和识别不同类型的细胞。神经谱系标记抗体装配试剂盒极具性价比，包含了最具代表性的神经谱系标记抗体，包括神经Stem cells和祖细胞标记Nestin、成熟神经元标记NeuN和MAP2、未成熟神经元标记TUBB3、放射状胶质细胞标记Vimentin、成熟星形胶质细胞和其他胶质细胞标记GFAP。这些抗体在多种应用和物种中进行了测试。有关抗体具体使用信息，请参阅各个抗体产品数据表。
Product Picture	Primary: Anti-MAP2 (SL1369R) Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP2) Polyclonal Antibody, Unconjugated (SL1369R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Primary: Anti-TUBB3 (Neuronal Marker) (SL4512R) Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TUBB3/beta III Tubulin(Neuronal Marker) Polyclonal Antibody, Unconjugated(SL4512R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Primary: Anti- GFAP (SL0199R) Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GFAP Polyclonal Antibody, Unconjugated(SL0199R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Primary:Anti-NeuN(SL1613R) Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (SL1613R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Primary: Anti- Vimentin (SL0756R) Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (SL0756R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Primary: Anti-Nestin Polyclonal Antibody, Unconjugated(SL0008R) Primary:Anti-NeuN(SL1613R) Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (SL1613R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Primary: Anti-TUBB3 (Neuronal Marker) (SL4512R) Tissue/cell: BV-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TUBB3) Polyclonal Antibody, Unconjugated (SL4512R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. Primary: Anti- Vimentin (SL0756R) Tissue/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (SL0756R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. Primary: Anti-MAP2 (SL1369R) Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP2) Polyclonal Antibody, Unconjugated (SL1369R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Primary: Anti- GFAP (SL0199R) Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by a conjugated secondary (SL0295G-Cy3) at [1:500] for 90 minutes and DAPI staining of the nuclei. Primary: Anti-Nestin Polyclonal Antibody, Unconjugated(SL0008R) Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nestin Polyclonal Antibody, Unconjugated(SL0008R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

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