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Mouse Anti-human CD71 antibody
Mouse Anti-human CD71 antibody
Transferrin receptor protein 1; Transferrin receptor; CD71; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; TFRC; Transferrin receptor (p90 CD71); TRFR; TFR1_HUMAN.
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Details

Product Name human CD71
Chinese Name 小鼠抗人CD71单克隆抗体
Alias Transferrin receptor protein 1; Transferrin receptor; CD71; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; TFRC; Transferrin receptor (p90 CD71); TRFR; TFR1_HUMAN.  
literatures
Specific References  (1)     |     SLM-30051M has been referenced in 1 publications.
[IF=1.18] Ma Caiyun. et al. In Vitro Culture and Multipotency Evaluation of Broiler Umbilical Cord Mesenchymal Stem Cells. BRAZ ARCH BIOL TECHN. 2023 Feb;66:  IF,FCM ;  Human.  
Immunogen Species Mouse
Clonality Monoclonal
Clone NO. 7B4
React Species Human, 
Applications Flow-Cyt=1ug/test ICC=1:100 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 74/85kDa
Detection molecular weight 95
Cellular localization The cell membrane Secretory protein 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human Transferrin receptor 
Lsotype Mouse IgG1, k
Purification Affinity purified by Protein G
Buffer Solution 0.01M TBS(pH=7.4)
Storage Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

SWISS:
P02786

Gene ID:
7037

Product Picture
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CD71) monoclonal Antibody, Unconjugated (SLM-30051M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:K562.
Primary Antibody (green line): Mouse Anti-human CD71 antibody (SLM-30051M)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line):Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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