Product Name	WWOX
Chinese Name
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	F11B6
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-1:2000 IHC-P=1:100-500 Flow-Cyt=1:25 ICC=1:50 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	作为Tumour抑制因子，在Apoptosis中发挥作用。可能与p53/TP53协同作用以控制基因毒性应激诱导的细胞死亡。在TGFB1信号传导和TGFB1介导的细胞死亡中发挥作用。也可能在Tumour坏死因子（TNF）介导的细胞死亡中发挥作用。抑制Wnt信号，可能是通过在细胞质中隔离DVL2。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: MCF-7, 2: Mouse ovary, 3: Rat brain Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 47 kDa Observed MW: 47 kDa Tissue: Human cerebrum Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 4°C overnight Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60630R Tissue: Human testis Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60630R Tissue: Rat cerebrum Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60630R Cell line: A549 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for SLM-60630R Cell line: MCF-7 Fixation: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary Ab dilution: 1:50 Secondary Ab: Goat Anti-Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for SLM-60630R

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