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Product Name Integin alpha 3 Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. G11E8 React Species (predicted: Human, Mouse, ) Applications WB=1:1000-1:5000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml Lsotype IgG1/Kappa Purification Affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 整合素α-3/β-1是纤维连接蛋白、层粘连蛋白、胶原、表面活性物质、血小板反应蛋白和CSPG4的受体。整合素α-3/β-1以胶原依赖的方式为FAP(seprase)在侵袭足质膜上提供对接位点,因此可能参与粘附、侵袭形成和基质降解过程,促进细胞侵袭。α-3/β-1可能通过LGALS3介导CSPG4对endothelial cells迁移的刺激。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 4°C overnight
Lysate: A549
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 120 kDa
Observed MW: 130 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Lysate: HeLa, C2C12
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 120 kDa
Observed MW: 130 kDa
Tissue: Human adenocarcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60363M
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