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Product Name DBR1 Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. C11A2 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:200-1:1000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The nucleus Form Liquid Concentration 1mg/ml Lsotype IgG2b/Kappa Purification Affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail RNA-lariat脱支酶,或DBR1,在切除的lariat内含子RNA的分支点特异性水解2-prime-to-5-prime支链磷酸二酯键并将其转化为线性分子。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4°C overnight
Lysate: 1: Rat heart, 2: Mouse heart, 3: HeLa, 4: A431
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 75 kDa
Observed MW: 75 kDa
Tissue: Rat brain
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60354M
Tissue: Mouse brain
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60354M
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