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Product Name CALD1 Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. E12E3 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-1:1000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The cell membrane Form Liquid Concentration 1mg/ml Lsotype IgG2b/Kappa Purification Affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 肌动蛋白和肌球蛋白Binding protein,参与调节平滑肌和非肌细胞中的肌动球蛋白相互作用(可作为肌球蛋白和肌动蛋白丝之间的桥梁)。刺激肌动蛋白结合原肌球蛋白,增加肌动蛋白丝结构的稳定性。在肌肉组织中,通过结合F-肌动蛋白抑制肌动球蛋白ATP酶。钙调素减弱了这种抑制作用,而原肌球蛋白增强了这种抑制作用。与肌动蛋白、肌球蛋白、两种原肌球蛋白分子和钙调素相互作用。在细胞有丝分裂和受体封顶过程中也起着重要作用。参与周围神经再生过程中雪旺细胞的迁移。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Lysate: HeLa
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 75 kDa
Observed MW: 80 kDa
Tissue: Rat stomach
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60332M
Tissue: Mouse stomach
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60332M
Tissue: Human uterus smooth muscle
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60332M
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