Product Name	STING1
Chinese Name	Transmembrane protein173Recombinant rabbit monoclonal anti
Alias	Endoplasmic reticulum interferon stimulator; Mediator of IRF3 activation; Mitochondrial mediator of IRF3 activation; N terminal methionine proline tyrosine serine plasma membrane tetraspanner; Stimulator of interferon genes; Stimulator of interferon genes protein; TM173_HUMAN; Transmembrane protein 173; ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING; TMEM173; STING-beta.
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	H9B4
React Species	(predicted: Human, )
Applications	WB=1:500-1:2000 IHC-P=1:100-500 ICC=1:50 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] SWISS: Q86WV6 Gene ID: 340061 Database links: Entrez Gene: 340061 Human SwissProt: Q86WV6 Human STING由379个氨基酸构成，有数个跨膜结构域，在多种endothelial cells、epithelial cells中以及造血肝细胞均有表达。STING参与并促进DNA介导的先天免疫反应，即来自病毒和细菌的非CpG双链DNA与cGAS蛋白结合后被催化成第二信使cGAMP；接着二聚化的STING与cGAMP结合，发生构象变化，通过自噬小体（autophagsome）经内质网和高尔基体进一步进入细胞质，期间被Ubiquitin化后募集TBK1蛋白，再被磷酸化，之后与IRF3结合；接着IRF3被磷酸化后入核，诱导表达IFNs，cell factor、T细胞募集因子等进而增强机体的免疫能力，更大限度的杀伤细菌，病毒等病原体。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: THP-1, Raw264.7, HL-60 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 42 kDa Observed MW: 36 kDa Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STING1) Monoclonal Antibody, Unconjugated (SLM-60326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STING1) Monoclonal Antibody, Unconjugated (SLM-60326R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining. Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60326R Tissue: Human colon cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution:1:1000 Primary incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60326R Cell line: THP-1 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 1 hour at room temperature Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60326R

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