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Product Name ALDH1A1 Chinese Name 乙醛脱氢酶1型Recombinant rabbit monoclonal anti Alias cytosolic; Acetaldehyde dehydrogenase 1; AHD2; Ahd-2; AL1A1_HUMAN; ALDC; Aldehyde dehydrogenase 1 family member A1; Aldehyde dehydrogenase 1 soluble; Aldehyde dehydrogenase 1A1; Aldehyde Dehydrogenase; Aldehyde dehydrogenase cytosolic; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase liver cytosolic; ALDH 1; ALDH 1A1; ALDH class 1; ALDH-E1; ALDH1 A1; ALDH1; ALDH11; ALHDII; MGC2318; PUMB1; RALDH 1; RalDH1; Retinal dehydrogenase 1; ALDEHYDE DEHYDROGENASE A1; Aldehyde Dehydrogenase Family 1, Subfamily A1. Research Area Tumour Cell biology immunology transcriptional regulatory factor Immunogen Species Rabbit Clonality Monoclonal Clone NO. AH12 React Species Human, Mouse, Applications WB=1:500-2000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ALDH1A1 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011].
SWISS:
P00352
Gene ID:
216
Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Lysate: HepG2, Raji, Jurkat, K562, A549, Mouse liver
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 55 kDa
Observed MW: 55 kDa
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Mouse Kidney tissue lysates
Primary: Anti-ALDH1A1 (SLM-60321R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 46 kDa
Tissue: Mouse kidney Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60321RTissue: Human liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60321R
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