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Rabbit Anti-Keratin 16 antibody
Rabbit Anti-Keratin 16 antibody
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Product Name Keratin 16
Chinese Name
Immunogen Species Rabbit
Clonality Monoclonal
Clone NO. F12G2
React Species (predicted: Human, )
Applications WB=1:1000-1:5000 IHC-P=1:100-500 ICC=1:50 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Cellular localization cytoplasmic The cell membrane 
Form Liquid
Concentration 1mg/ml
Lsotype IgG
Purification Affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail 表皮特异性Ⅰ型角蛋白在皮肤中起关键作用。作为天然免疫的调节者,对皮肤屏障破坏做出反应:需要一些炎症检查点来维持皮肤屏障。
Product Picture
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 2 hours at room temperature
Lysate: HeLa
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 51 kDa
Observed MW: 51 kDa
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60319R
Tissue: Human esophagus
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60319R
Cell line: A431
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for SLM-60319R

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