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Product Name Keratin 16 Chinese Name Immunogen Species Rabbit Clonality Monoclonal Clone NO. F12G2 React Species (predicted: Human, ) Applications WB=1:1000-1:5000 IHC-P=1:100-500 ICC=1:50 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml Lsotype IgG Purification Affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 表皮特异性Ⅰ型角蛋白在皮肤中起关键作用。作为天然免疫的调节者,对皮肤屏障破坏做出反应:需要一些炎症检查点来维持皮肤屏障。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 2 hours at room temperature
Lysate: HeLa
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 51 kDa
Observed MW: 51 kDa
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60319R
Tissue: Human esophagus
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for SLM-60319R
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