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Rabbit Anti-CK-7 antibody
Rabbit Anti-CK-7 antibody
Cytokeratin 7; Cytokeratin-7; CK 7; CK-7; K7; Keratin 7; Keratin,type II cytoskeletal 7; KRT7; SCL; K2C7_HUMAN.
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Details

Product Name CK-7
Chinese Name 细胞角蛋白7Recombinant rabbit monoclonal anti
Alias Cytokeratin 7; Cytokeratin-7; CK 7; CK-7; K7; Keratin 7; Keratin,type II cytoskeletal 7; KRT7; SCL; K2C7_HUMAN.  
Research Area Signal transduction  b-lymphocyte  
Immunogen Species Rabbit
Clonality Monoclonal
Clone NO. C2G6
React Species Human, 
Applications WB=1:500-2000 IHC-P=1:200-1:1000 ICC=1:50 IF=1:50-100 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 54kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human CK-7 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

SWISS:
P08729

Gene ID:
3855

Database links:

Entrez Gene: 3855 Human

SwissProt: P08729 Human



Product Picture
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Lysate: HeLa, MCF-7, A549
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 51 kDa
Observed MW: 50 kDa
Tissue: Human ovarian carcinoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60305R
Tissue: Human breast cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60305R
Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60305R

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