Product Name	Caldesmon
Chinese Name	钙介质素Recombinant rabbit monoclonal anti
Alias	CAD; CALD 1; CALD1; Caldesmon 1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; aldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; Caldesmon1; CDM; H CAD; HCAD; L CAD; LCAD; MGC21352; NAG22; Caldesmon-Pan; CALD1_HUMAN.
Research Area	Cardiovascular  Signal transduction  marrow cells
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	C2D4
React Species	Human, Mouse, Rat,
Applications	WB=1:500-1000 IHC-P=1:1000 ICC=1:50-100 IF=1:50-100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	94kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Caldesmon
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jul 2008]. SWISS: Q05682 Gene ID: 800 Database links: Entrez Gene: 800 Human Entrez Gene: 109624 Mouse Entrez Gene: 204926 Rat SwissProt: Q05682 Human SwissProt: Q8VCQ8 Mouse SwissProt: Q05682 Rat
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Lysate: C2C12 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 93 kDa Observed MW: 75 kDa Tissue: rat colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60289R Tissue: Human colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60289R Tissue: Mouse colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60289R Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60289R

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