Product Name	CD21
Chinese Name	CR2Recombinant rabbit monoclonal anti
Alias	C3DR; CD 21; CD21 antigen; Complement C3d receptor; Complement component (3d/Epstein Barr virus) receptor 2; Complement component receptor 2; Complement receptor type 2; Cr 2; Cr2; EBV receptor; Epstein Barr virus receptor; CR2_HUMAN.
Research Area	Tumour  Signal transduction  Cytoskeleton  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	CD12B9
React Species	Human,
Applications	WB=1:500-2000 IHC-P=1:1000 ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	111kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD21
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] SWISS: P20023 Gene ID: 1380 Database links: Entrez Gene: 1380 Human SwissProt: P20023 Human
Product Picture	Sample: Lane 1: Human Raji cell lysates Lane 2: Human Raji cell lysates Primary: Anti-CD21 (SLM-60246R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kDa Observed band size: 150 kDa Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD21) Monoclonal Antibody, Unconjugated (SLM-60246R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Cell line: Raji Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:100 Primary incubation condition: 1 hour at room temperature Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60246R

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