Product Name	CD22
Chinese Name	Blymphocyte粘附分子CD22Recombinant rabbit monoclonal anti
Alias	B-cell receptor CD22; B cell receptor CD22 precursor; B lymphocyte cell adhesion molecule; BL CAM; BL-CAM; BLCAM; CD 22; CD22 antigen; CD22 molecule; CD22 protein; Leu14; Lyb8; MGC130020; Sialic acid binding immunoglobulin like lectin 2; SIGLEC 2; SIGLEC2; SIGLEC-2; FLJ22814; MGC130020; CD22_HUMAN; B-lymphocyte cell adhesion molecule; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14.
Research Area	Tumour  immunology  Stem cells  Cell adhesion molecule  Cell Surface Molecule  Cell type markers  lymphocyte  b-lymphocyte
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	G12H9
React Species	Human,
Applications	WB=1:500-2000 IHC-P=1:1000 ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	91kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD22
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome. [provided by Alliance of Genome Resources, Nov 2021] SWISS: P20273 Gene ID: 933 Database links: Entrez Gene: 933 Human SwissProt: P20273 Human
Product Picture	Sample: Lane 1: Human Raji cell lysates Lane 2: Human Raji cell lysates Primary: Anti-CD22 (SLM-60233R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91 kDa Observed band size: 135 kDa Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Lysate: Daudi Protein loading quantity: 20 μg Exposure time: 15 s Predicted MW: 95 kDa Observed MW: 130 kDa Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60233R Tissue: Human spleen Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60233R Cell line: Daudi Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:100 Primary incubation condition: 1 hour at room temperature Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60233R

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