Product Name	MSH6
Chinese Name	错配修复蛋白6Recombinant rabbit monoclonal anti
Alias	DNA mismatch repair protein MSH6; G/T mismatch binding protein; GTBP; GTMBP; HNPCC 5; HNPCC5; HSAP; MSH 6; mutS (E. coli) homolog 6; MutS alpha 160 kDa subunit; mutS homolog 6; p160; Sperm associated protein; MSH6_HUMAN; MutS Protein Homolog 6.
Research Area	Chromatin and nuclear signals
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	M5F6
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 IHC-P=1:200-1:1000 ICC=1:100 IF=1:50-100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	153kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MSH6
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013] SWISS: P52701 Gene ID: 2956
Product Picture	Sample: Lane 1: Human HeLa cell lysates Lane 2: Human 293T cell lysates Lane 3: Human SH-SY5Y cell lysates Primary: Anti-MSH6 (SLM-60220R) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 153 kDa Observed band size: 180 kDa Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:4000 Primary ab incubation condition: 2 hours at room temperature Lysate: HeLa, N2a Protein loading quantity: 20 μg Exposure time: 15 s Predicted MW: 150 kDa Observed MW: 170 kDa Tissue: Human colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60220R Tissue: Rat testis Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60220R Tissue: Human colon cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60220R Tissue: Mouse liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for SLM-60220R Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:100 Primary incubation condition: 1 hour at room temperature Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for SLM-60220R

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