TEL: +86 571 56623320 EMAIL: [email protected]
Product Name 2-Hydroxyisobutyryl-Histone H3 (Lys14) Chinese Name Immunogen Species Rabbit Clonality Monoclonal Clone NO. B3E9 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-1:2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The nucleus Form Liquid Concentration 1mg/ml Lsotype IgG Purification Affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 组蛋白受到多种酶催化修饰,包括乙酰化、甲基化、磷酸化和Ubiquitin化等。赖氨酸2-羟基异丁基酰化是一种新发现的控制染色体结构和基因转录的可逆修饰,其可逆性已在从蠕虫到人类的真核组蛋白中得以证实。组蛋白2-羟基异丁基酰化的独特结构和基因组定位表明,它在机制和功能上与组蛋白乙酰化有所不同。具体而言,在人类体细胞和小鼠雄性生殖细胞基因组中,组蛋白2-羟基异丁酰化标记了活性启动子或潜在的增强子。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+ sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+ sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+ sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Scan Wechat Qrcode