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Product Name β-Hydroxybutyryl-Histone H3 (Lys9) Chinese Name Immunogen Species Rabbit Clonality Monoclonal Clone NO. E6A5 React Species (predicted: Human, Rat, ) Applications WB=1:500-1:2000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The nucleus Form Liquid Concentration 1mg/ml Lsotype IgG Purification Affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 组蛋白能被多种酶催化发生修饰,比如乙酰化,甲基化,磷酸化和Ubiquitin化等。赖氨酸β-羟基丁酰化(Kbhb)是近期发现的一种主要富集在活性基因启动子区的组蛋白标记。 研究表明,在培养细胞时,提高β-羟基丁酸盐浓度;长期禁食或用链脲佐菌素诱导小鼠Diabetes酮症,可显著提高细胞或小鼠肝脏中的Kbhb水平。 组蛋白Kbhb是一种可以将代谢与基因表达偶联的全新表观遗传调控机制,为研究染色质调控以及一些重要生理病理疾病比如Diabetes,癫痫和Tumour中β-羟基丁酸盐的多种功能提供了新途径。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium 3-ohbutyrylate (50mM, 72hr)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Tissue: Rat colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60206R
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