Product Name	Ubiquityl-Histone H2B (Lys120)
Chinese Name
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	A12B4
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-1:1000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG2a/Kappa
Purification	Antigen affinity purification
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	核小体将DNA包裹并压缩成染色质，限制了DNA进入需要DNA作为模板的细胞机制。因此，组蛋白在转录调控，DNA修复，DNA复制和染色体稳定性等方面起着重要作用。组蛋白H2B第120位赖氨酸单Ubiquitin化是一种重要的组蛋白修饰，在许多物种的基因转录调控和高级染色质组织中起着重要作用。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa, HepG2, Neuro-2a Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 23 kDa Observed MW: 23 kDa Tissue: Human liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60166M Tissue: Rat liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60166M Tissue: Mouse kidney Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60166M

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