Product Name	Histone H3 (Tri Methyl K36)
Chinese Name	三甲基化组蛋白H3抗体
Alias	H3K36me3; Tri-Methyl-Histone H3 (Lys36); Histone H3(Tri Methyl Lys36); Tri methyl Histone H3(Lys36); Tri methyl Histone H3(K36); H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3.1; H3/d; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD;
Product Type	Methylated anti Recombinant rabbit monoclonal anti
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	B3C9
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-1:2000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of Tri Methyl K36
Lsotype	IgG
Purification	Affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. SWISS: P68431 Gene ID: 8350 组蛋白翻译后修饰（PTMs）是Epigenetics调控染色质结构的关键机制，被称为“组蛋白密码”。组蛋白上的翻译后修饰包括乙酰化，甲基化，磷酸化和近年发现的一些新型酰化修饰。这些组蛋白修饰直接影响染色质和转录因子或其他表观调控子的结合，改变基因组的稳定性和基因转录等。组蛋白甲基化通常发生在核心组蛋白的赖氨酸和精氨酸残基。组蛋白甲基化既可以促进也可以抑制基因转录，这取决于组蛋白甲基化是发生在赖氨酸还是发生在精氨酸上以及甲基化基团的个数（赖氨酸可以发生一、二、三甲基化，精氨酸可发生单、对称与非对称二甲化）。组蛋白赖氨酸甲基化通常发生在组蛋白H3第4，9，27，36，79和组蛋白H4的第20位赖氨酸上；精氨酸甲基化通常发生在组蛋白H3的第2，8，17，26和组蛋白H4第3位精氨酸上。蛋白甲基化酶（HMT）和组蛋白去甲基化酶（HDM）是甲基化修饰主要的调控因子。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1. MCF-7, 2. NIH-3T3, 3. HepG2, 4. Rat kidney, 5. Mouse kidney, 6. Recombinant Histone H3 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa Tissue: Human colon cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60113R Tissue: Mouse stomach Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60113R Tissue: Rat kidney Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60113R

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