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Product Name Phospho-Histone H3 (Ser10) Chinese Name 磷酸化组蛋白H3单克隆抗体 Alias Histone H3 (phospho S10); Phospho-Histone H3(Ser10); Histone H3(Phospho-Ser10); Histone H3(p-Ser10); Histone H3(Phospho-S10); H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H31_HUMAN. Product Type Phosphorylated anti Immunogen Species Mouse Clonality Monoclonal Clone NO. C10C6 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-1:1000 IHC-P=1:100-500 ICC=1:50-1:100 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 15kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10: RK(p-S)TG Lsotype IgG1/Kappa Purification Affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Subcellular Location:
Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC).
SWISS:
P68431
Gene ID:
8350
组蛋白翻译后修饰(PTMs)是Epigenetics调控染色质结构的关键机制,被称为“组蛋白密码”。组蛋白上的翻译后修饰包括乙酰化,甲基化,磷酸化和近年发现的一些新型酰化修饰。这些组蛋白修饰直接影响染色质和转录因子或其他表观调控子的结合,改变基因组的稳定性和基因转录等。组蛋白磷酸化通常发生在核心组蛋白N端的丝氨酸,苏氨酸或酪氨酸上,在DNA修复,转录和染色质折叠等方面起着重要作用。广为人知的组蛋白磷酸化位点为H2A.xS139ph,有文献报道此位点与DNA损伤相关。组蛋白磷酸化主要参与有丝分裂和减数分裂过程。参与调控磷酸化水平的激酶与磷酸酶多种多样。Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+ Nocodazole (100ng/ml, 18hr)
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Tissue: Rat testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60080M
Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60080M
Tissue: Human kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60080M
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:100
Primary incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for SLM-60080M
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